姓名:胡晓敏
职务/职称:教授
通讯地址:永利集团88304官网生命科学学院8号楼8C303
E-mail:xiaomin.hu@mail.scuec.edu.cn
研究领域和方向:
主要从事农业和食品微生物的分子遗传与应用研究。其中基础研究包括芽胞杆菌和噬菌体的分子遗传结构和进化、基因调控、代谢和功能蛋白的特性分析等;应用研究包括芽胞杆菌杀蚊剂的研发和应用,动物养殖业噬菌体/衍生酶抗菌剂的研发和应用。
教育背景:
2002.09 – 2007.03,中国科学院武汉病毒研究所,微生物学专业,获博士学位
2002.09-2003.09/2005.05-2005.10: 丹麦奥胡斯大学国立环境调查研究所,访问学生
1996.09 – 2000.06,华中师范大学,生物化学专业,获学士学位
工作简历:
2019.11–至今:永利集团88304官网
2007.03 – 2019.11:中国科学院武汉病毒研究所
2007/11-2009/09:比利时天主教鲁汶大学
主持的主要科研项目情况:
纵向项目
1. 2022-2025,国家自然科学基金面上项目“球形赖氨酸芽胞杆菌“整合可动员型”杀蚊基因岛GI8与接合转移质粒pBsph的互作”,负责人。
2. 2023-2024,国家自然科学基金中比国际合作项目“携杀蚊毒素基因的整合型接合转移因子的鉴定和特性分析”,负责人。
3. 2022-2024,武汉市科技知识创新项目“裂解酶表达文库的构建及新型裂解酶抗菌剂的筛选和优化”,负责人。
4. 2022-2024,陕西省重点研发计划“针对仔猪、犊牛和羔羊的功能性饲料添加剂产品和应用技术的研发与示范”,负责人。
5. 2018-2020,国家科技重大专项“一带一路”传染病防控保障关键技术中“东非、中亚和东南亚等区域重要传染病防控技术”,子课题负责人。
6. 2017-2020,农业部两减项目“棉花害虫生防微生物流行病学”,子课题科研骨干。
7. 2016-2020 , “一带一路”新发突发病原研究、子课题科研骨干。
8. 2016-2019,国家自然科学基金面上项目“球形芽胞杆菌ICEBsph1的鉴定及对杀蚊毒素基因binA/binB的水平传播分析”,负责人。
9. 2016-2018, 国家重点研发项目“针对 ZIKV、DENV、CHIKV的传播媒介伊蚊的防控产品及技术”,子课题科研骨干。
10. 2013-2018, 非洲病原微生物及流行病防控、子课题科研骨干。
11. 2012-2015,国家自然科学基金面上项目“蜡状芽胞杆菌群呕吐毒素基因簇的水平转移机理研究”,负责人。
12. 2009-2011,国家自然科学基金青年基金项目“杀蚊球形芽胞杆菌的遗传分型及毒素基因进化”,负责人。
横向项目
1. 2019-2021 动物养殖常见病原菌的检测和抗菌剂的研发、负责人。
2. 2020-2021 生物杀蚊幼制剂的研制和应用推广、负责人。
第一作者和通讯作者论文(*通讯):
1) Wan X, Mahillon J, Yuan Z, Zhang G, Geng P, Hu X*. Characterization of vB_SalS_PSa2, a T5-like Demerecviridae bacteriophage, and its potential use in food matrices. Virology. 2024 Aug;596:110101.
2) Geng P, Zhao P, Wan X, Mahillon J, Hu Y, Gong Y, Hu X*. Interspecies horizontal transfer and specific integration of the mosquitocidal toxin-encoding plasmid pTAND672-2 from Bacillus thuringiensis subsp. israelensis to Lysinibacillus sphaericus. Appl Environ Microbiol. 2023 Feb 28;89(2):e0165222.
3) Wang Y, Zhao P, Zhou Y, Hu X*, Xiong H*. From bitter to delicious: properties and uses of microbial aminopeptidases. World J Microbiol Biotechnol. 2023 Jan 10;39(3):72. doi: 10.1007/s11274-022-03501-3
4) Peiling Geng, Yunfei Gong, Xiaofu Wan, Xiaomin Hu*. Influence of the phagemid pfNC7401 on cereulide-producing Bacillus cereus NC7401. Microorganisms. 2022 Apr 30;10(5):953.
5) Zhao P, Zhang M, Wan X, Geng P, Xiong H*, Hu X*. Characterization and heterologous expression of a novel Co(2+)-dependent leucyl aminopeptidase Amp0279 originating from Lysinibacillus sphaericus. Appl Microbiol Biotechnol. 2022 Feb;106(3):1139-1149.
6) Zhang X, Meng H, Hu X*, Yuan Z*. Diversity and functional profile of gut symbiotic bacteria between Lysinibacillus sphaericus C3-41 susceptible and resistant Culex quinquefasciatus Say as revealed by 16S rRNA gene high-throughput sequencing. Front Microbiol. 2022 Nov 3:13:991105.
7) Geng P, Cheng J, Yuan Z, Xiong H, Wang H, Hu X*. Horizontal transfer of large plasmid with type IV secretion system and mosquitocidal genomic island with excision and integration capability in Lysinibacillus sphaericus. Environ. Microbiol. 2021 Sep;23(9):5131-5146.
8) Wan X, Geng P, Sun J, Yuan Z*, Hu X*. Characterization of two newly isolated bacteriophages PW2 and PW4 and derived endolysins with lysis activity against Bacillus cereus group strains. Virus Res. 2021 Sep 16;198489.
9) Hu X#*, Huang D#, Ogalo J#, Geng P, Yuan Z, Xiong H, Wan X, Sun J. Application of Bacillus thuringiensis strains with conjugal and mobilizing capability drives gene transmissibility within Bacillus cereus group populations in confined habitats. BMC Microbiol. 2020 Nov 26;20(1):363.
10) Zhang X, Karungu S, Cai Q, Yuan Z*, Hu X*. Effects of propoxur exposure on insecticidal susceptibility and developmental traits in Culex pipiens quinquefasciatus. Insects. 10(9). 288. 2019. Sep
11) Karungu S, Atoni E, Ogalo J, Mwaliko C, Agwanda B, Yuan Z*, Hu X*. Mosquitoes of Etiological Concern in Kenya and Possible Control Strategies. Insects. 10(6):173. 2019. Jun
12) Tian S, Xiong H, Geng P, Yuan Z*, Hu X*. CesH Represses Cereulide Synthesis as an Alpha/Beta Fold Hydrolase in Bacillus cereus. Toxins. 11, 231. 2019.
13) Fu P, Ge Y, Hu Y, Yuan Z*, Hu X*. A toxin-antitoxin system is essential for the stability of mosquitocidal plasmid pBsph of Lysinibacillus sphaericus. Microbiol Res. 2018 Sep; 214:114-122.
14) Geng P, Wan X, Cheng J, Yuan Z*, Hu X*. vB_LspM-01: A novel myovirus displaying pseudolysogeny in Lysinibacillus sphaericus C3-41. Appl Microbiol Biotechnol. 2018 Dec;102(24):10691-10702.
15) Hu Y, Cai Q, Tian S, Ge Y, Yuan Z*, Hu X*. Regulator DegU is required for multicellular behavior in Lysinibacillus sphaericus. Res Microbiol. 2018 Apr;169(3):177-187.
16) Fu P, Ge Y, Wu Y, Zhao N, Yuan Z*, Hu X*. The LspC3-41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3-41. BMC Microbiol. 17(1):116. 2017.
17) Fu P, Xiang X, Ge Y, Yuan Z, Hu X*. Differential expression of duplicated binary toxin genes binA/binB in Lysinibacillus sphaericus C3-41. Lett Appl Microbiol. 65(1):90-97. 2017.
18) Geng P, Hu Y, Zhou G, Yuan Z*, Hu X*. Characterization of three autolysins with activity against cereulide-producing Bacillus isolates in food matrices. Int J Food Microbiol. 241:291-297. 2017.
19) Xu K, Yuan Z, Rayner S*, Hu X*. Genome comparison provides molecular insights into the phylogeny of the reassigned new genus Lysinibacillus. BMC Genomics. 16:140. 2015.
20) Shi T, Ge Y, Zheng D, Zhao N, Cai Q, Hu X*, Yuan Z*. Polyphosphate kinase of Lysinibacillus sphaericus and its effects on accumulation of polyphosphate and bacterial growth. Micriobiol. Res.172:41-7. 2015.
21) Mei X, Xu K, Yang L, Yuan Z, Mahillon J, Hu X*. The genetic diversity of cereulide biosynthesis gene cluster indicates a composite transposon Tnces in emetic Bacillus weihenstephanensis. BMC Microbiol. 14:149. 2014.
22) Ge Y1, Hu X1, Zheng D, Wu Y, Yuan Z*. Allelic Diversity and Population Structure of Bacillus sphaericus Revealed by Multilocus Sequence Typing. Appl Environ Microbiol. 77 (5): 5553–5556. 2011.
23) Hu X, Swiecicka I, Timmery S, Mahillon J*. Sympatric soil communities of Bacillus cereus sensu lato: population structure and potential plasmid dynamics of pXO1- and pXO2-like elements. FEMS Microbiol. Ecology. 70: 344-355. 2009.
24) Hu X, Van der Auwera G, Timmery S, Zhu L, Mahillon J*. Distribution, diversity, and potential mobility of extrachromosomal elements related to the Bacillus anthracis pXO1 and pXO2 virulence plasmids. Appl Environ Microbiol. 75: 3016-3028. 2009.
25) Hu X, Fan W, Han B, Liu H, Liu D, Zheng D, Li Q, Yan J, Berry C, Yuan Z*. Complete genome sequences of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with closely related Bacillus species. J Bacteriol. 190: 2892-2902. 2008.
26) Hu X, Li J, Hansen BM, Yuan Z*. Phylogenetic analysis and heterologous expression of surface layer protein SlpC of Bacillus sphaericus C3-41. Biosci Biotech Bioch. 72: 1257-1263. 2008.
27) Hu X, Hansen BM, Hendriksen NB, Yuan Z*. Detection and phylogenic analysis of one anthrax virulence plasmid pXO1 conservative open reading frame ubiquitous presented within Bacillus cereus group strains. Biochem Biophysi Res Commu. 349: 1214-1219. 2006.
28) Hu X, Hansen BM, Johansen JE, Hendriksen NB, Smidt L, Jensen GB, Yuan Z*. Transfer and expression of the mosquitocidal plasmid pBtoxis in Bacillus cereus group strains. FEMS Microbiol Lett. 245: 239-247. 2005.
29) Hu X, Hansen BM*, Eilenberg J, Hendriksen NB, Smidt L, Yuan Z, and Jensen GB. Conjugative transfer, stability and expression of a plasmid encoding a cry1Ac gene in Bacillus cereus group strains. FEMS Microbiol Lett. 231: 45-52. 2004.
30) 胡晓敏; 蔡亚君; 周帼萍; 袁志明*。蜡状芽胞杆菌群菌株中部分病原相关因子的检测。微生物学报, 2007, 47(3): 392~395。
31) 张小磊;袁志明;胡晓敏*。 致倦库蚊对杀虫剂的抗性现状及治理. 《中国媒介生物学及控制杂志》 2019年30(06):719-724。
32) 孙佳慧、耿佩玲、万小芙、袁志明、熊海容,胡晓敏*。噬菌体吸附及受体结合蛋白(RBP)的研究进展与应用。《生物工程学报》。2021,37(8):2614-22.
出版著作(*通讯)
1) Hu X, Mahillon J*. Chapter 5: Life cycle and gene exchange. Endospore-forming soil bacteria, Soil Biology, Edited by N. A Logan & P. De Vos. Spriger-Verlar Berlin Heidelberg, pp 89-114, Germany. 2011.
2) 胡晓敏,袁志明*。中国微生物基因组研究. 主编喻子牛,科学出版社。2012。撰写章节页码P307-331。
参编教材:
《生物安全导论》, 科学出版社,“十四五”普通高等教育研究生规划教材,2024年6月第一版。
教学活动:
1. 本科生理论课程:《生物信息学》、《合成生物学》
2. 本科生实验课程:《生物工程大实验》《发酵工程及设备模块》《分离技术模块》